estrogen receptor alpha antibody

Anti-Estrogen Receptor-α Antibody detects endogenous levels of total Estrogen Receptor-α protein. The connection between estrogenic and PI3K/AKT/mTOR signaling pathways is frequently associated with poor outcome in ER-positive breast tumors. Anti-Estrogen Receptor α Antibody is a Rabbit Polyclonal Antibody for detection of Estrogen Receptor α also known as Estrogen receptor-like 1, Estrogen-related receptor alpha & has been validated in EMSA, ICC, IHC, IP & WB. We have examined expression of the classic estrogen receptor, estrogen receptor-alpha (ERalpha), during osteoclast differentiation. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Estrogen nuclear receptor alpha antibody. Estrogen Receptor antibody LS-C26682 is an unconjugated mouse monoclonal antibody to Estrogen Receptor (ER Alpha) from human. Immunogen Range: 136-185 Physical form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. If you are interested in the product for IVD use, please click here. The TE111.5D11 clone was developed against the entire ER ligand binding … In addition, the expression of ERRα has been shown to increase during fasting and cold exposure. This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. Select up to 5 products from below to compare or request more information. Be the first to share your experience with this product. Products (1468) Write a Review; Company View; Product View; Your search returned 1468 Estrogen Receptor alpha Antibodies across 46 suppliers. Immunohistochemistry (IHC) techniques involve the selective identification of antigen proteins by exploiting these antigen-antibody relationships to characterize your analyte of interest. ESR1 Reactivity: Human FACS, IHC, StM Host: Mouse Monoclonal ESR1-3559 camera_alt 4 Catalog No. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Anti-phospho-Estrogen Receptor alpha (Ser118) Antibody, clone NL44, rabbit monoclonal. Anti-mouse IgG HRP-linked antibody (7076S) and anti-rabbit IgG HRP-linked antibody (7074S) were purchased from Cell Signalling Technology (Hamilton House, Mabledon Place, London, WC1H 9BB, UK). ABIN6939347. Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERα), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. IHC staining of purified anti-Estrogen Receptor antibody (clone CO1531) on formalin-fixed paraffin-embedded human breast tumor tissue. ER is an important regulator of growth and differentiation in the mammary gland. A300-497A. Anti-Estrogen Receptor alpha Antibody Products. Estrogen Receptor alpha (Y537) Antibody, ALEXA FLUOR® 555 Conjugated. It is reactive with human and mouse. Rabbit Estrogen Receptor alpha Rabbit pAb (A0296), validaed in WB and tested in Human,, Mouse. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Conjugations made fresh to order. Nuclear hormone receptor. View specifications, prices, citations, reviews, and more. Validated for IHC, IP and WB. Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen Receptor (ER) is a rabbit monoclonal antibody (RMab) for immunohistochemistry from Bio SB. Western blot analysis of whole cell lysates probed with estrogen receptor alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 3 second exposure.Arrow points to estrogen receptor alpha … 927901), the tissue was incubated with a 1:40 dilution of the primary antibody for 60 min at room temperature. Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). - Find MSDS or SDS, a COA, data sheets and more information. Western blot analysis of Estrogen Receptor alpha on different lysates. ABclonal provides free trial antibodies for target detection. Rabbit Monoclonal Antibody to Estrogen Receptor -Alpha is affinity purified and diluted in PBS, pH 7.6, containing 1% BSA and 0.09% sodium azide. Journal of Dairy Science 86 (Suppl. Qty -+ Interested in a bulk order or custom quote? ESTRR antibody. 1) 118. Zhou D, Li S, Wang X, Cheng B, Ding X. Estrogen receptor alpha is essential for the proliferation of prostatic smooth muscle cells stimulated by 17β-estradiol and insulin-like growth factor 1. Estrogen receptor alpha (ER alpha) and ER beta are members of the steroid nuclear receptor family that modulate gene transcription in an estrogen-dependent manner. Estrogen Receptor alpha Antibody (pSer104) - Rabbit polyclonal antibody to human ER-alpha. DEPTOR is an mTORC1/2 inhibitory protein that is degraded in response to mitogen … Accessories. 100% Guaranteed. Dilution of Primary Antibody BioGenex Ready-to-Use antibodies have been optimized for use with the recommended BioGenex Detection System and should not require further dilution. There are currently no references for Estrogen Receptor alpha antibody (GTX50420). Please allow a delivery lead time of up to 3 weeks. Multiple p … Clinical significance of estrogen receptor phosphorylation Endocr Relat Cancer. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. Validated for WB, IHC, ELISA with human, mouse, and rat tissue. Estrogen receptor antibody. Pavao M(1), Traish AM. To characterize this connection, we performed a phosphoproteome analysis of ER-positive MCF7 cells. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. DEPTOR was identified as an estrogen-regulated mTORC1 target. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Western blot analysis of extracts from untransfected MCF-7 cells, and COS-1 cells transfected with wild-type or mutant ER alpha, stimulated with EGF and E2, using Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb (upper) or control estrogen receptor alpha antibody #2512 (lower). Showing 28 of 46 suppliers (1468 products total) 1; 2 > >> Select All. ER Alpha / Estrogen Receptor estrogen receptor 1. Estrogen receptor antibodies: specificity and utility in detection, localization and analyses of estrogen receptor alpha and beta. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. 100 µl (1000 µg/ml) $367 100 µl (1000 µg/ml) A300-497A-T. 10 µl (1000 µg/ml) $103 10 µl (1000 µg/ml) Request Quote for Bulk Quantity. Estrogen related receptor alpha (ERRα) is a nuclear receptor in the superfamily of ligand-regulated transcription factors and is a member of the NR3B orphan nuclear receptor subgroup (consisting of α, β and γ). Request Info for all products Compare. 100ul. Now with the emerging availability of phospho-site-specific antibodies to ERα, the relevance of phosphorylation of ERα in human breast cancer in vivo is being explored. Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11) Catalog Number MA5-13065 Product Data Sheet Product Specific Information MA5-13065 targets Estrogen Receptor in ChIP, GS, IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Hamster, Human, mouse, Ovine, Porcine, Rabbit, and Rat samples. Submit a Reference REVIEW. ERα is a ~65 kDa steroid hormone receptor containing an N-terminal ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an domain. 2011;29:120-5 pubmed publisher Major ORF antibody. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Cell Biochem Funct. NR3A1 antibody. This Estrogen Receptor alpha antibody is conjugated to PerCP Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB) Specificity Detects ~66 kDa. estrogen receptor alpha E1-E2-1-2 antibody; estrogen receptor alpha E1-N2-E2-1-2 antibody; ESTRR antibody; NR3A1 antibody; nuclear receptor subfamily 3 group A member 1 antibody; oestrogen receptor alpha antibody; Ordering Information. anti-Estrogen Receptor alpha anticorps (Estrogen Receptor 1) (AA 129-312) Primary Antibody ESR1 Reactivité: Humain FACS, IHC, StM Hôte: Souris Monoclonal ESR1-3559 tamoxifen) therapy. anti-Estrogen Receptor alpha antibody (Estrogen Receptor 1) (AA 129-312) Primary Antibody. $402. Multiple sites of phosphorylation on human estrogen receptor α (ERα) have been identified by a variety of methodologies. Compare Anti-Estrogen Receptor-alpha Antibody Products from leading suppliers on Biocompare. Anti-estrogen receptor alpha antibody (ab16660) was purchased from Abcam (330 Cambridge Science Park, Cambridge, CB4 0FL, UK). ER mRNA and protein have been detected both peripherally and in the central nervous system, with most data having come from the rat. The estrogen receptor test (ERT) uses the estrogen receptor (ER) tumor marker that allows for immunohistochemical techniques to be performed for diagnostic purposes. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. Reactivité: Humain, Souris Hôte: Lapin Clone: Polyclonal Conjugué: Cy3 | Commandez Estrogen Receptor alpha + beta l'anticorps (ABIN725858). Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody (GTX127978) diluted at 1:2000 and competitor's antibody (MA1-310) diluted at 1:100. Nuclear receptor subfamily 3 group A m ember 1 antibody : Protein information supplied by UniProt . The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha. Connor EE, Capuco AV, Sonstegard TS, Mota AF, Wood DL, Garrett W, Bennett GL & Williams J 2003a Quantitative analysis of estrogen-related receptor alpha, estrogen receptor alpha and estrogen receptor beta mRNAs throughout bovine mammary gland development. ERRα plays a role in modulating the estrogen signaling pathway. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. There are currently no reviews for Estrogen Receptor alpha antibody (GTX50420). Estrogen receptor is a nuclear receptor for estrogens such as estradiol (the main endogenous human estrogen). Be the first to share your publications with this product. No. Author information: (1)Department of Biochemistry, Center for Advanced Biomedical Research, Boston University School of Medicine, 700 Albany Street, W-607, Boston, MA 02118, USA. Estrogen receptor 1 antibody.

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